Motherwort fruit medicinal material specific chromatogram construction method and detection method

The invention relates to a motherwort fruit medicinal material specific chromatogram construction method and detection method. The specific chromatogram construction method comprises the following steps: preparing a reference solution and a test solution; detecting the reference substance solution a...

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Bibliographische Detailangaben
Hauptverfasser: WEI MEI, WU WENPING, DENG CONGYOU, LI GUOWEI, WU SHIRONG, CHEN XIANGDONG, HE MINYOU, CHENG XUEREN, WU SHUZHEN, SUN DONGMEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a motherwort fruit medicinal material specific chromatogram construction method and detection method. The specific chromatogram construction method comprises the following steps: preparing a reference solution and a test solution; detecting the reference substance solution and the test solution by adopting ultra-high performance liquid chromatography, wherein a mobile phase adopted by the ultra-high performance liquid chromatography is as follows: acetonitrile is used as a mobile phase A, and a trifluoroacetic acid aqueous solution with the volume concentration of 0.05-0.15% is used as a mobile phase B; and adopting gradient elution. According to the characteristic spectrum construction method, the characteristic spectrum of the motherwort fruit medicinal materialwith multiple common peaks is constructed, and the motherwort fruit medicinal material can be comprehensively, effectively and quickly evaluated. 本发明涉及一种茺蔚子药材的特征图谱构建方法和检测方法。所述特征图谱构建方法包括如下步骤:对照品溶液和供试品溶液制备;采用超高效液相色谱法对所述对照品溶液和供试