Cucumber fruit thorn nodule paraffin section manufacturing method
The invention discloses a cucumber fruit thorn nodule paraffin section manufacturing method which comprises the following steps: (1) selecting a cucumber fruit thorn nodule material in a proper development period, immediately putting the cucumber fruit thorn nodule material into a container bottle f...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a cucumber fruit thorn nodule paraffin section manufacturing method which comprises the following steps: (1) selecting a cucumber fruit thorn nodule material in a proper development period, immediately putting the cucumber fruit thorn nodule material into a container bottle filled with an FAA fixing solution, slightly shaking the container bottle for at least one time, andfixing the container bottle for 30 minutes after the cucumber fruit thorn nodule material sinks on the liquid level; (2) completely pouring out the FAA stationary liquid in the container bottle, adding a new FAA stationary liquid into the container bottle again, sealing a bottle opening with a sealing film, and fixing the container bottle for at least 48 hours; (3) completely pouring out the FAA stationary liquid in the container bottle again, adding 50% ethanol into the container bottle, and performing inducing for 1 hour; and (4) finally, sequentially carrying out finishing dyeing, dehydration, transparency, waxing |
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