Application of TGFBI as marker for regulating osteogenic differentiation of mesenchymal stem cells

The invention discloses an application of TGFBI in preparation of a product for regulating mesenchymal stem cell osteogenic differentiation. Experiments show that the TGFBI gene secreted by human umbilical cord derived mesenchymal stem cells can regulate osteogenic differentiation of the mesenchymal stem cells; specifically, a lentivirus transfection technology is utilized to introduce a synthesized TGFBI lentivirus into human umbilical cord derived mesenchymal stem cells subjected to subculture; by knocking down the TGFBI gene, the expression of an early marker alkaline phosphatase (ALP) fordifferentiation and maturation of osteoblasts and an advanced marker osteopontin (OPN) for differentiation and maturation of osteoblasts is significantly up-regulated, thereby promoting the osteogenicdifferentiation capacity of huMSC. 本发明公开了TGFBI在制备调控间充质干细胞成骨分化的产品中的应用。本发明人通过实验发现人脐带来源的间充质干细胞分泌的TGFBI基因可以调节间充质干细胞成骨分化；具体地，利用慢病毒转染技术将合成的TGFBI慢病毒导入传代培养后的人脐带来源的间充质干细胞，通过敲低TGFBI基因使得成骨细胞分化成熟的早期标志物碱性磷酸酶(ALP)和成骨细胞分化成熟的晚期标志物骨桥蛋白(OPN)的表