Preparation method of platelet-rich cytokine plasma freeze-dried powder

The invention provides a preparation method of platelet-rich cytokine plasma freeze-dried powder. The preparation method comprises the following steps: repeatedly freezing and thawing platelet-rich plasma in liquid nitrogen to obtain cytokine-rich plasma; pre-freezing at the temperature of -80 DEG C; and then putting into a vacuum freeze dryer pre-cooled at-45 DEG C, and freeze-drying according toa set freeze-drying procedure to obtain freeze-dried powder. The method for activating platelets to release cytokines is a liquid nitrogen repeated freezing and thawing method, the platelets are activated thoroughly, and the amount of the released cytokines is large; the content of the cytokines in the freeze-dried powder is basically the same as that of the cytokines in a sample before freeze-drying, and the freeze-drying method provided by the invention can ensure the activity of the cytokines to the greatest extent. 本发明提供了一种富血小板细胞因子血浆冻干粉的制备方法：先将富血小板血浆置于液氮中反复冻融，获得富细胞因子血浆；再于-80℃预冻；然后放入-45℃预冷的真空冷冻干燥机内，按照设定冻干程序进行冻干，获得