Application of protein 14-3-3 h1 in TMV infected tobacco leaves

The invention provides application of a coding gene 14-3-3 h1 of a tobacco protein 14-3-3 h1 in defense of TMV infected tobacco. The gene 14-3-3 h1 has a sequence shown in SEQ ID No. 1. The tobacco protein 14-3-3 h1 is applied to the defense of the TMV infected tobacco through inhibiting a vesicle transferring promoting function of a protein Arf to inhibit replication of TMV in cells. According tothe application, a detailed process for infecting tobacco by the TMV is researched from molecular biology and genetic engineering, a safe and effective infection prevention and control method is explored under the condition of being independent of chemical control drugs, and the aim of further carrying out early-stage warning and control on tobacco mosaic disease is achieved. 本发明提出了烟草14-3-3 h1蛋白的编码基因14-3-3 h1在防御TMV侵染烟草中应用，14-3-3 h1基因序列为SEQ ID NO.1；烟草14-3-3 h1蛋白通过抑制Arf蛋白促进囊泡转运功能抑制TMV在细胞内的复制，在防御TMV侵染烟草中应用。本发明从分子学和基因工程上研究TMV侵染烟草的详细过程，在不依赖化控药物使用的情况下，寻求一种安全有效的防控感染方法，达到进一步对烟草花叶病进行早期预警和防治的目的。