Qualitative detection method for pioglitazone and rosiglitazone in food and medicine

The invention provides a qualitative detection method for pioglitazone and rosiglitazone in foods and medicines and belongs to the field of substance detection. The method is characterized in that twotypes of gold nanoparticles are proportioned to serve as nanoparticle enhancing reagent, water-saturated trichloromethane serves as extracting agent for extraction, acid is not needed for extraction,the method is safer, interference is reduced, the Raman effect of a target object is enhanced, the characteristics are more obvious and the strength is higher under the same concentration, and then accuracy of the detection result can be improved. Data of the embodiment show that the method is suitable for detecting the illegally added pioglitazone and the rosiglitazone in the hypoglycemic health-care food by surface enhanced Raman spectroscopy, and detection limits of the method are both 15 mu g/mL. 本发明提供了一种食品和药品中吡格列酮和罗格列酮的定性检测方法，属于物质检测领域。本发明采用两种金纳米粒子配比作为纳米粒子增强试剂，辅以水饱和三氯甲烷作为提取剂进行萃取，不用酸进行提取，更安全，且使干扰减少，目标物的拉曼效应得到增强，在相同