Immunofluorescence staining method for paraffin embedded three-dimensional suspension cell clusters

The invention relates to an immunofluorescence staining method of paraffin embedded three-dimensional suspension cell clusters. The method comprises the following steps: pretreating the three-dimensional suspension cell clusters, immersing into paraffin liquid to embed sections, carrying out antigen repair, and carrying out immunofluorescence staining. According to the method provided by the invention, cells in the cell cluster are fully dyed, the result is accurate, the overall protein expression and distribution condition of the suspension cell cluster can be observed, and sample observationcan be carried out only by a common fluorescence microscope. 本发明涉及一种石蜡包埋三维悬浮细胞团的免疫荧光染色方法，该方法包括如下步骤：对三维悬浮细胞团进行预处理后，浸入石蜡液中包埋切片，进行抗原修复，再进行免疫荧光染色。本发明提供的方法细胞团内部细胞染色充分，结果准确，可以观察悬浮细胞团整体的蛋白表达和分布情况，且仅需要普通的荧光显微镜即可进行样本观察。