Magnetic bead sorting liquid for DNA separation, and preparation method thereof

The invention provides a magnetic bead sorting liquid for DNA separation. 51 ml of the magnetic bead sorting liquid includes 1 ml of GE magnetic beads, 5-10 g of PEG, 12.5-50 mg of Tween 20, 50-125 mmol of NaCl, 0.25-1 mmol of Tris, 0.025-0.1 mmol of EDTA and 0.085-0.34 mmol of HCl. The invention also provides a preparation method for preparing the magnetic bead sorting liquid. The preparation method takes the GE magnetic beads as a raw material, comprises a GE magnetic bead cleaning step and a GE magnetic bead resuspending step, and has the advantages of simplicity and stability in operation,short consumed time and low cost. The prepared magnetic bead sorting liquid has the functions of DNA purification and fragmentation screening, can be kept stable for a long time, does not need further chemical modification, improves the DNA separation and purification efficiency, can replace imported magnetic beads, and is suitable for large-scale library building production. 本发明提供一种用于DNA分离的磁珠分选液，以51ml体积的该磁珠分选液计，包含1ml GE