Peony type 2S albumin and extraction method and application thereof

The invention discloses peony type 2S albumin and an extraction method and application thereof. The amino acid sequence of the peony type 2S albumin is as shown in SEQID NO.1. Peony seeds or peony seed meal is used as a raw material, through degreasing treatment, performing leaching with a PBS buffe...

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Hauptverfasser: SHEN YEHUA, CHEN BANG, LI CONG, YANG JUZHUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses peony type 2S albumin and an extraction method and application thereof. The amino acid sequence of the peony type 2S albumin is as shown in SEQID NO.1. Peony seeds or peony seed meal is used as a raw material, through degreasing treatment, performing leaching with a PBS buffer solution, performing coarse extraction of protein by an ammonium sulfate precipitation method, andperforming DEAE anion exchange and chromatographic purification, the peony type 2S albumin is obtained. The peony type 2S albumin disclosed by the invention is simple in extraction technology, the purity of the obtained peony type 2S albumin can reach 95% or above, the peony type 2S albumin has higher homology with a 2S albumin family, and besides, the obtained peony type 2S albumin has favorablereducing capacity on FRAP, has favorable eliminating capacity on ABTS free radicals, hydroxyl free radicals and DPPH free radicals, exhibits better antioxidant activity and can be used as an antioxidant. 本发明公开了一种牡丹类2S白蛋白及其提取方