CHROMATOGRAPHY COLUMN WITH LOCKED PACKED BED AND METHOD OF PACKING THAT COLUMN

A chromatography column has a retaining plug permanently fixed to an upstream end of the column and blocks one end of the bore through the column. The plug has a fluid passage therethrough. An upstream end of the passage is preferably but optionally larger in diameter than a downstream end of the passage. An upstream porous member upstream of the retaining plug is held by an upstream end cap and urged toward the plug. Chromatographic media extends from the upstream porous member, through the passage in the retaining plug, to a downstream porous member held by a downstream end cap. The media between the retaining plug and the downstream porous member are under compression to form a bed of packed media. 色谱柱具有保持塞，该保持塞永久地固定至柱的上游端并阻塞穿过柱的孔的一端。保持塞具有穿过其中的流体通道。该通道的上游端的直径优选但任选地大于通道的下游端的直径。保持塞的上游的上游多孔部件由上游端盖保持并被推向该塞。色谱介质从上游多孔部件通过保持塞中的通道延伸到由下游端盖保持的下游多孔部件。保持塞和下游多孔部件之间的介质受到压缩以形成填充介质的床。