Extraction-free SARS-CoV-2 nucleic acid detection method and kit

The invention provides an extraction-free method and a kit for qualitatively detecting SARS-CoV-2 by fluorescence polymerase chain reaction (fluorescence PCR), and belongs to the field of biotechnology and medicine. The method integrates sample nucleic acid release and amplification. The kit comprises a lysis solution and a rapid enzyme, can directly lyse and amplify respiratory secretions of a patient, make a whole detection process fast and simple, reduce chance of mutual contamination, obtain accurate and reliable PCR test results, can diagnose presence of SARS-CoV-2 in patient secretions as soon as possible, and has important application value in the fields of disease monitoring and clinical diagnosis. 本发明提供一种免提取荧光聚合酶链反应(荧光PCR)定性检测新型冠状病毒的方法和试剂盒，属于生物技术与医学领域。本发明的方法将样本核酸释放与扩增融为一体，试剂盒中包含了裂解液和快速酶，可以直接对病人呼吸道分泌物进行裂解与扩增，使整个检测过程快速，简便，减少相互污染的机会，获得的PCR检测结果准确可靠，能够尽快确诊病人分泌物中是否存在新型冠状病毒，在疾病监测、临床诊断等领域具有重要的应用价值。