Glycyl proline dipeptidylaminopeptidase determining reagent kit

The invention discloses a glycyl proline dipeptidylaminopeptidase determining reagent kit, and relates to the technical field of biologic detection. The glycyl proline dipeptidylaminopeptidase determining reagent kit comprises a reagent R1 and a reagent R2 which are mutually independent, wherein the...

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Bibliographische Detailangaben
Hauptverfasser: SHANG YIWEN, ZHANG XIAOQING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a glycyl proline dipeptidylaminopeptidase determining reagent kit, and relates to the technical field of biologic detection. The glycyl proline dipeptidylaminopeptidase determining reagent kit comprises a reagent R1 and a reagent R2 which are mutually independent, wherein the reagent R1 comprises the following ingredients by corresponding content of 5-10g/L trihydroxymethylaminomethane, 5-10g/L glycylglycine and 0.1-0.5g/L sodium azide, and the reagent R2 comprises the following ingredients by corresponding content of 10-20g/L glycyl proline para-nitroaniline tosylate,20-80ml/L ethanediol, 2-8g/L bovine serum albumin and 0.1-0.5g/L sodium azide. In an implementation process, the addition proportion of mannitol to mannan to glucosamine in the reagent R2 is (1-5) to(1-2) to 1, besides, the volume ratio of the reagent R1 to the reagent R2 is controlled to be (5-10) to 1, stability and accuracy of the reagents are reinforced, the capacity of resisting disturbanceof the reagents is increase