Calf bos taurus chymosin of high activity and high yield and gene, strain and application

The invention belongs to the technical field of gene engineering and relates to calf bos taurus chymosin of a high activity and a high yield and a gene, a strain and application. The amino acid sequence of the chymosin BoCHY provided by the invention is shown in SEQ ID NO:1. The chymosin BoCHY provi...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG LIUQUN, LIU GUIZI, YANG JIANGKE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE ENZYMOLOGY FOODS OR FOODSTUFFS HUMAN NECESSITIES MAKING THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MILK OR CHEESE SUBSTITUTES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of gene engineering and relates to calf bos taurus chymosin of a high activity and a high yield and a gene, a strain and application. The amino acid sequence of the chymosin BoCHY provided by the invention is shown in SEQ ID NO:1. The chymosin BoCHY provided by the invention is obtained through artificial orientated transformation on the basis of calf bos taurus chymosin Chy, and has remarkably increased activities and expression levels. 本发明属于基因工程技术领域，涉及一种高活性高产的小牛皱胃凝乳酶及基因与菌株和应用。本发明的凝乳酶BoCHY的氨基酸序列如SEQ ID NO：1所示。本发明所述凝乳酶BoCHY为在小牛皱胃凝乳酶Chy的基础上通过人工定向改造而来，具有显著提高的活性和表达水平。