Cell culture solution and preparation method and application thereof

The invention discloses a cell culture solution and a preparation method and application thereof. The cell culture solution comprises a culture solution, penicillin sodium, streptomycin and cephalosporin antibiotics, wherein the working concentration of the penicillin sodium in the cell culture solu...

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Bibliographische Detailangaben
Hauptverfasser: WANG ZHIQIANG, ZHU YANPING, YE LINLU, ZHU XUEZHEN, HAO YANPENG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a cell culture solution and a preparation method and application thereof. The cell culture solution comprises a culture solution, penicillin sodium, streptomycin and cephalosporin antibiotics, wherein the working concentration of the penicillin sodium in the cell culture solution is 0.01 to 0.06 mg/ml, the working concentration of the streptomycin in the cell culture solution is 0.01 to 0.1 mg/ml, and the working concentration of the cephalosporin antibiotics in the cell culture solution is 0.01 to 0.1 mg/ml. When being used for culturing HELA cells, the cell culture solution not only has a prevention effect on pathogenic bacteria, but also has a wider antibacterial and bacteriostatic range, and can kill gram-positive anaerobic bacteria and gram-negative anaerobic bacteria, so that the HELA cells are prevented from being polluted by the pathogenic bacteria. The culture environment is more beneficial to the growth of the HELA cells, so that the growth speed of the HELA cells is increase