Method for knocking off myo7ab gene of zebra fish

The invention relates to the technical field of gene knockoff and in particular relates to a method for knocking off the myo7ab gene of zebra fish. According to the method, an appropriate targeting site is designed on the myo7ab gene of zebra fish by using a CRISPR/Cas9 (clustered regularly interspaced shortpalindromic repeats-associated 9) gene edition technique, and specific sgRNA (small guide ribonucleic acid) and Cas-9mRNA (messenger ribonucleic acid) are synthesized in vitro for gene edition of the zebra fish. By adopting the method, specific genes can be efficiently and precisely silenced, in addition, the preparation is simple, the cost is low, multiple sites on the target gene can be sheared simultaneously, and any number of single genes can be silenced. The invention further provides a method for knocking off genes to breed myo7ab gene deleted zebra fish. The zebra fish model constructed by using the method is helpful in further revealing the whole process of generation of otic bulla and capillary ce