Culture medium for human mesenchymal stem cells

The invention provides a culture medium for human mesenchymal stem cells. The culture medium for human mesenchymal stem cells (hMSC) includes a mesenchymal stem cell basal medium; human leucocyte/platelet coat lysate (buffy coat); insulin; sodium selenite; ethanolamine; and basic fibroblast growth f...

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Hauptverfasser: DIEZ CERVANTES JOSE MARIA, GAJARDO RODRIGUEZ RODRIGO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a culture medium for human mesenchymal stem cells. The culture medium for human mesenchymal stem cells (hMSC) includes a mesenchymal stem cell basal medium; human leucocyte/platelet coat lysate (buffy coat); insulin; sodium selenite; ethanolamine; and basic fibroblast growth factor (bFGF). This culture medium is effective for growing hMSC lines, including those which do notgrow in culture medium normally used for this type of cell. 本发明涉及用于人间充质干细胞的培养基。更具体地,本发明涉及其中hMSC细胞系能够生长的培养基,包括在通常用于这类细胞的培养基中不能生长的那些细胞系。所述用于人间充质细胞的培养基包括通常用于培养间充质干细胞的基础培养基;人白细胞/血小板层溶解产物(血沉棕黄层);胰岛素;亚硒酸钠;乙醇胺;以及碱性成纤维细胞生长因子(bFGF)。