Separating and purifying method of apolipoprotein A-II from serum

The present invention discloses a separating and purifying method of apolipoprotein A-II from serum. The separating and purifying method of the apolipoprotein A-II from serum comprises the following steps: conducting preliminary purification of an apolipoprotein A-II component in serum by using a hydrophobic chromatography packing material, washing impurities with a salt-free buffer solution, conducting elution with a buffer solution containing organic alcohol, collecting an eluent containing the A-II apolipoprotein, using an anion exchange chromatography packing material to finely purify thecollected eluent containing the A-II apolipoprotein, using a buffer solution containing a detergent and organic alcohol to perform degreasing on column of the apolipoprotein A-II, etc. The method onlyobtains the apolipoprotein A-II with purity larger than or equal to 95% through 2 steps of the chromatography and has a recovery rate larger than or equal to 50%. 本发明公开了一种从血清中分离纯化载脂蛋白A-II的方法，包括利用疏水层析填料对血清中的载脂蛋白A-II成分进行初步提纯，用不