Method for detecting bovine fat traits by utilizing SDC3 promoter genetic marker

The invention discloses a method for detecting bovine fat traits by utilizing an SDC3 promoter genetic marker. By carrying out PCR amplification on whole genome DNA of cattle and by detecting SNPs existing in a PCR product sequence by DNA sequencing, the invention discloses the correlation between s...

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Bibliographische Detailangaben
Hauptverfasser: BAI ZITONG, XIA LIXIN, SUN HAO, YU XIANG, YANG RUNJUN, YU HAIBIN, FANG XIBI, ZHAO ZHIHUI, MI JIAQI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for detecting bovine fat traits by utilizing an SDC3 promoter genetic marker. By carrying out PCR amplification on whole genome DNA of cattle and by detecting SNPs existing in a PCR product sequence by DNA sequencing, the invention discloses the correlation between seven SNPs sites on an SDC3 gene promoter region and bovine fat traits; and the method for predictingand breeding marbling grade, fat coverage rate and fat color character of beef cattle by utilizing the SDC3 gene promoter region marker lays a foundation for early breeding of genetic workers, improvement of meat quality and further improvement of the economic benefit of beef cattle breeding. 本发明公开了一种利用SDC3启动子遗传标记检测牛脂肪性状的方法,通过对牛的全基因组DNA进行PCR扩增后,通过DNA测序的方法检测PCR产物序列存在的SNPs,公开了SDC3基因启动子区上的7个SNPs位点与牛脂肪性状的相关性;利用SDC3基因启动子区标记作为预测和选育肉牛大理石花纹等级、脂肪覆盖率及脂肪颜色性状的方法,为遗传工作者的早期选育及改善肉品质从而进一步提高肉牛养殖的经济效益奠定基础。