Detection primer pair, kit and method for sheep GHR gene insertion/deletion polymorphism and application of method
The invention relates to a primer pair for detecting sheep growth hormone receptor (GHR) gene insertion/deletion polymorphism. The primer pair can be used for PCR amplification of a fragment containing a sheep GHR gene intron region insertion/deletion polymorphic site. The method can detect the geno...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a primer pair for detecting sheep growth hormone receptor (GHR) gene insertion/deletion polymorphism. The primer pair can be used for PCR amplification of a fragment containing a sheep GHR gene intron region insertion/deletion polymorphic site. The method can detect the genotype of the insertion/deletion polymorphic site simply, quickly, at low cost and accurately, and thedetected insertion/deletion polymorphic site can be used as a molecular marker site for the number of lambs produced by sheep, so that the establishment of sheep populations with excellent litter size traits can be accelerated, and the speed of improved variety breeding can be improved.
本发明涉及一种绵羊GHR基因插入/缺失多态性检测用引物对,所述引物对能够用于PCR扩增包含绵羊GHR基因内含子区插入/缺失多态性位点的片段。本发明能够简单、快速、低成本、精确地检测所述插入/缺失多态性位点的基因型,检测的插入/缺失多态性位点能够作为绵羊产羔数的分子标记位点,从而加快建立产羔数性状优良的绵羊种群,提高良种选育速度。 |
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