Detection method for insertion/deletion polymorphism of sheep RORA (retinoid acid receptor related orphan receptor A) gene, primer pair and application

The invention relates to a detection method for insertion/deletion polymorphism of a sheep RORA (retinoid acid receptor related orphan receptor A) gene. The detection method comprises the following steps: by taking genome DNA (deoxyribonucleic acid) of sheep to be detected as a template, and a primer pair as amplification primers, performing PCR (polymerase chain reaction) amplification on a fragment with insertion/deletion polymorphic sites in a sheep RORA gene intron region, performing electrophoresis on an amplification product, and identifying genotypes of the insertion/deletion polymorphic sites according to electrophoresis results, wherein the insertion/deletion polymorphic sites are selected from 23-bp insertion/deletion polymorphic sites of NC_040258.1:S.10400_10423 sites of the sheep RORA gene. By adopting the method, the genotypes of the insertion/deletion polymorphic sites can be simply, rapidly and precisely detected with low cost. 本发明涉及一种绵羊RORA基因插入/缺失多态性的检测方法，步骤如下：以待测绵羊基因组DNA为模板，以引物对为扩增引物，利用PCR扩增