Method for determining RNA containing poly(A) and kit

The invention relates to the field of biological information, and specifically relates to a method for determining RNA containing poly(A) and a kit. The method includes the steps of connecting a 3' adaptor to the 3' end of the RNA containing poly(A) so as to obtain RNA containing the 3' adaptor; taking the RNA containing the 3' adaptor as a template to perform reverse transcription, connecting a 5' adaptor to the 5' end of the RNA containing the 3' adaptor so as to obtain RNA containing the 3' adaptor and the 5' adaptor; and constructing sequencing library based on the RNA containing the 3' adaptor and the 5' adaptor so as to perform determination on the RNA containing the poly(A). Through the utilization of the method, the information of full-length RNA and the poly(A) can be obtained without interrupting; and the method is simple and convenient in operation step. 本发明涉及生物信息领域，具体涉及一种对含有poly(A)的RNA测定的方法及试剂盒。该方法包括在所述含有poly(A)的RNA的3'末端，连接3'接头，以便获得含有3'接头的RNA；以所述含有3'接头的RNA为模板进行反转录，在所述含有3'接头的RNA的5'末端连接5'接头，以便获得含有3'