Improved counting method of living bacteria

The invention discloses an improved counting method of living bacteria. The improved counting method of the living bacteria comprises the following steps: (1) sterilizing a sand-core filter and a microporous filter membrane used for performing filtration, assembling the sterilized sand-core filter and microporous filter membrane, and putting the assembled sand-core filter and microporous filter membrane into a super clean bench for standby application; (2) uniformly oscillating a diluted sample solution by using a vortex mixer so as to obtain a uniformly mixed diluted sample solution; (3) performing suction filtration on the uniformly mixed diluted sample solution by using the assembled sand-core filter and microporous filter membrane, slowly attaching the microporous filter membrane to amedium, carrying out light-shielded cultivation, and carrying out immobilization by using formaldehyde so as to obtain an immobilized filter membrane; (4) dyeing the immobilized filter membrane by using acridine orange, placi