Method for detecting content and related substances of flumethasone

The invention provides a method for detecting the content and related substances of flumethasone. A Sursil C18 silica gel chromatographic column is used, acetonitrile and water are used as mobile phases, a gradient elution mode is adopted, the detection wavelength is 230-240nm, the flow velocity is 0.5 ml/min to 1.0 ml/min; and the column temperature is 25-35 DEG C. The detection method disclosedby the invention can be used for accurately and effectively separating seven analogue impurities contained in the flumethasone, has good specificity and accuracy, and is suitable for detecting the flumethasone serving as a raw material. Methodological verification shows that the method can be used for accurately controlling the process of flumethasone and degrading impurities, and can be used forguiding the investigation of a stable sample. 本发明提供一种双氟美松含量和有关物质的检测方法，采用Spursil C18硅胶色谱柱，以乙腈和水为流动相，采用梯度洗脱的方式，检测波长选自230nm-240nm；流速为0.5ml/min-1.0ml/min；柱温为25～35℃。本申请中的检测方法可准确、有效分离双氟美松所含7种类似物杂质，具有良好的专属性及准确性，适用于原料双氟美松检测。经方法学验证后表明，