Creatine kinase isozyme assay kit

The invention discloses a creatine kinase isozyme assay kit, belonging to the technical field of biological detection. The kit comprises a reagent R1 and a reagent R2 which are independent of each other. The reagent R1 comprises the following components: imidazole acetate, D-glucose, magnesium acetate L, hexokinase, N-acetylcysteine, adenosine diphosphate (ADP), an oxidized coenzyme II (NADP+), sodium azide, ethylenediamine tetraacetic acid (EDTA), glucose-6-phosphate dehydrogenase, adenosine 5'-disodium monophosphate (AMP), and diadenosine pentaphosphate (P1P5-diAP). The reagent R2 comprisesthe following components: creatine phosphate, sodium azide, and an anti-human CK-M antibody. In the implementation process, the stability, accuracy and precision of the kit are obviously improved by controlling the concentration ratio of all the components within a range disclosed by the invention, and the kit is simple to operate, low in preparation cost and suitable for promotion and application. 本发明公开了一种肌酸激酶同工酶测定试剂盒，涉及