Novel GDSL family lipid hydrolase EII-3, and coding gene and application thereof

The invention discloses a marine bacterium-derived novel metal ion-resistant GDSL family hydrolase EII-3, and a coding gene and an application thereof. The hydrolase gene is derived from a marine bacterium Alterobacter gangjensis JCM 17802T, and the nucleotide sequence of the hydrolase gene is represented by SEQ ID NO. 1. After heterologous expression of the hydrolase gene, the hydrolase has esterase activity, and can maintain high enzymatic activity in the presence of an organic solvent and metal ions. The esterase EII-3 encoded by the gene has excellent metal ion resistance, so that the esterase EII-3 can be applied to industrial production under salt-containing and various metal ion conditions such as wastewater treatment, fine chemical engineering, pharmacy and environmental remediation. 本发明公开了一种海洋细菌来源新型耐金属离子GDSL家族水解酶EII-3及其编码基因与应用。本发明所涉及水解酶基因来自海洋细菌Altererythrobacter gangjinensis JCM 17802，核苷酸序列如SEQ ID NO.1所示。本发明所述的水解酶基因经异源表达后，该水解酶具有酯酶活性，在有机溶剂和金属离子存在的条件下，能保持较高的酶学活性。该基因所编码的酯酶EⅡ-3优良的耐金属离子特性，使其可应用于废水处理、精细化工、