Thermophilic anaerobic ethanol bacillus genome editing vector and application thereof

The invention belongs to the field of gene engineering, and particularly relates to a thermophilic anaerobic ethanol bacillus genome editing vector and application thereof. According to the thermophilic anaerobic ethanol bacillus genome editing vector provided by the invention, a thermostable Cas9 expression element sequence and/or an sgRNA expression element sequence are/is constructed into an escherichia coli-thermophilic anaerobic ethanol bacillus shuttle expression vector; the gene in-frame insertion or gene fragment deletion of the thermophilic anaerobic ethanolobacter genome can be realized. The genome of the thermophilic anaerobic ethanol bacillus is edited by using the vector provided by the invention to obtain a mutant engineering strain with gene deletion, so that a genome editing method of the thermophilic anaerobic ethanol bacillus is provided. 本发明属于基因工程领域，具体涉及一种嗜热厌氧乙醇杆菌基因组编辑载体及其应用。本发明提供的嗜热厌氧乙醇杆菌基因组编辑载体将热稳定的Cas9表达元件序列和/或sgRNA表达元件序列构建到以大肠杆菌-嗜热厌氧乙醇杆菌穿梭表达载体中；可实现嗜热厌氧乙醇杆菌基因组的基因框内插入或基因片段缺失。利用本发明所述的载体对嗜热