Method for detecting content of alpha-homonojirimycin in suregade glomerulata medicinal material

The invention provides a method for detecting the content of alpha-homonojirimycin in a suregade glomerulata medicinal material. The method comprises the following steps: preparing a reference substance solution, namely, precisely weighing an alpha-nojirimycin reference substance, and adding a solve...

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Bibliographische Detailangaben
Hauptverfasser: WU ZHENGJUN, DAI ZHI, YUAN QINFEN, LI QIONGYA, HAN XIAOQIANG, DUAN JUANHUI, LI WENYAN, XU BING, WANG YONG, WANG HONGQING, MA PENGGANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for detecting the content of alpha-homonojirimycin in a suregade glomerulata medicinal material. The method comprises the following steps: preparing a reference substance solution, namely, precisely weighing an alpha-nojirimycin reference substance, and adding a solvent for dissolving to obtain the reference substance solution with required concentration; preparinga test solution, namely, taking suregade glomerulata medicinal material powder to be detected, adding an extraction solvent for extracting, carrying out centrifuging, taking a supernatant, and fixingthe volume to obtain the test solution; and carrying out content determination, namely, determining the peak areas of the alpha-homonojirimycin in the reference substance solution and the test solution through a liquid chromatography-tandem mass spectrometry method, and calculating the content of the alpha-homonojirimycin in the suregade glomerulata medicinal material according to an external standard method. According to