Microbial fluorescent staining solution and application thereof
The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining solution can be used in microbial dyeing, specific recognition of agglutinin on glycoprotein and specific staining of nucleic acid by nucleic acid dye are utilized, according to the invention, the fungi are blue, green or red, the bacteria are red, the morphology is combined to achieve the detection on the microorganisms in a vaginal secretion sample, a cervical exfoliated cell sample, a skin sample and a sputum sample, the detection result can be visualized, and the microorganism variety can be directly and reasonably judged.
本发明涉及一种微生物荧光染色液及其应用。所述微生物荧光染色液包括凝集素、荧光素、核酸染料、缓冲溶液、抗淬灭剂、抑菌剂和水;可应用于微生物染色,其利用凝集素对糖蛋白的特异性识别和核酸染料对核酸的特异性染色,使得真菌呈现蓝色、绿色或者红色,细菌呈现红色,结合形态学实现对阴道分泌物样本、宫颈脱落细胞样本、皮肤样本以及痰液样本中微生物的检测,且可将检测结果可视化,更为直接、合理的对微生 |
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