Application of berberine in prevention and treatment of pulpitis

The invention discloses an application of berberine in prevention and treatment of pulpitis. Berberine can promote proliferation of LPS-HDPF cells, reduce the expression level of inflammatory factorsIL-1 beta, IL-6 and TNF-alpha, up-regulate the expression level of miR-21 and down-regulate the expression level of KBTBD7. Over-expression of miR-21 or silencing of KBTBD7 can enhance the promotion effect of BBR on LPS-HDPF cell proliferation, and significantly inhibit the inflammatory factor level. MiR-21 is used for carrying out targeted negative regulation on KBTBD7. In BBR treatment of LPS-HDPF cells, a miR-21 inhibitor promotes the phosphorylation level of IKK and p65 and inhibits the expression of I kappa B alpha protein, and silenced KBTBD7 inhibits the phosphorylation level of IKK andp65 and promotes the expression of I kappa B alpha protein. 本发明公开了小檗碱在防治牙髓炎中的应用。小檗碱能够促进LPS-HDPF细胞增殖，降低炎症因子IL-1β、IL-6和TNF-α的表达水平，且上调miR-21表达水平，下调KBTBD7的表达水平；过表达miR-21或沉默KBTBD7可加强BBR对LPS-HDPF细胞增殖的促进作用，显著性抑制炎症因子水平；miR-21靶向负调节KBT