Single-cell dispersion microfluidic chip, and preparation method and operation method thereof

The invention discloses a single-cell dispersion microfluidic chip. The single-cell dispersion microfluidic chip comprises a fluid shearing force dispersion module layer, a microstructure array dispersion module layer and an isolation adhesion layer between the fluid shearing force dispersion module layer and the microstructure array dispersion module layer; the fluid shearing force dispersion module layer is mainly used for carrying out single-cell dispersion on tissue blocks or tissue spheres with large sizes (100-2000 microns); and the microstructure array dispersion module layer is used for carrying out single-cell dispersion on tissue blocks or tissue spheres with small sizes (100 microns or below). The invention also discloses a preparation method and an operation method of the microfluidic chip, the preparation method and the operation method have the characteristics of simple and rapid operation and low consumption, and the single-cell dispersion microfluidic chip can be widelyapplied to various paral