Plant insect-resistant gene and vector and application of plant insect-resistant gene and vector

The invention discloses an artificially-synthesized Bt insecticidal gene mCry1Ab for transgenic insect-resistant plants and a protein for insect resistance of the plants. The amino acid sequence of the protein is SEQ ID NO: 1, and the nucleotide sequence of a coding gene of the protein is SEQ ID NO:...

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Bibliographische Detailangaben
Hauptverfasser:	LYU YUPING, LIU FENG, LI XIAOJIAO, ZHAO LIYUAN, LI SHUXIU, ZHANG YUAN, WANG QIANG, JIA ZHIWEI, LI TAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an artificially-synthesized Bt insecticidal gene mCry1Ab for transgenic insect-resistant plants and a protein for insect resistance of the plants. The amino acid sequence of the protein is SEQ ID NO: 1, and the nucleotide sequence of a coding gene of the protein is SEQ ID NO: 3. The invention also discloses applications of a corresponding vector, the protein and the gene ininsect pest resistance. The mCry1Ab gene modified by the invention solves the problems that the Cry1Ab gene is easy to break and poor in recombination and resistance, and has a better insect-resistant effect compared with a Cry1Ab gene. 本申请公开了一种人工合成用于转基因抗虫植物的Bt杀虫基因mCry1Ab以及用于植物抗虫的蛋白质，所述蛋白质的氨基酸序列为SEQ ID NO:1，其编码基因的核酸序列为SEQ ID NO 3。本申请还公开了相应的载体，以及蛋白质、基因在抗虫害中的应用。本申请改造后的mCry1Ab基因解决了Cry1Ab基因容易断裂，以及重组和抗性差的问题，并且相对于Cry1Ab基因具有更佳的抗虫效果。