Method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings

The invention discloses a method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings and belongs to the technical field of plant tissue culture. The method comprises the following step of inoculating degenerated or dormant common callalily tissue culture...

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Bibliographische Detailangaben
Hauptverfasser: WU HONGZHI, WU JINGZHI, ZHOU WEN, GUO YANBING, HE SHUILIAN, JIN SHOULIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method for regeneration, rejuvenation and dormancy breaking of common callalily tissue culture seedlings and belongs to the technical field of plant tissue culture. The method comprises the following step of inoculating degenerated or dormant common callalily tissue culture seedlings to a special culture medium, wherein the culture medium is prepared by preparing a motherliquor according to an MS basic culture medium formula, and adding 0.008-0.015mg/L of TDZ, 0.05-0.20mg/L of 6-Ba, 28-35g/L of sucrose and 6.20-6.50g/L of agar powder in the mother liquor, and a pH value of the culture medium is adjusted to be between 6.0 and 6.1. By adopting the method disclosed by the invention, the ability of restoring differentiation and robust growth of the degraded common callalily tissue culture seedlings can be promoted, dormancy of the common callalily is broken, the preservation period of the common callalily is prolonged, and the effective technical support is provided for preservation and