Fluorescent quantitative PCR kit for detecting rubella viruses

The invention discloses a fluorescent quantitative PCR kit for detecting rubella viruses. The kit comprises a PCR reaction solution, an internal standard, a positive quality control material and a negative quality control material, wherein the PCR reaction solution comprises an upstream primer, a downstream primer and a fluorescent probe which are used for detecting rubella virus RNA, and the internal standard material and the positive quality control material are respectively RNA pseudovirus containing chlamydia trachomatis tryptophan synthetase beta subunit encoding gene and RNA pseudoviruscontaining rubella virus specific conservative gene segment. The kit provided by the invention can detect all genotypes of rubella viruses published in rubella epidemiology in China, adopts pseudoviruses as internal standards, can truly and effectively carry out quality control on possible false negative detection results, and greatly improves the detection sensitivity, accuracy and stability. 本发明公开了一种检测风疹病毒的荧光定量PCR试剂盒，该试