Yeast hydrolysate with high free nucleotide and preparation method and application of yeast hydrolysate

The invention relates to the field of biological fermentation and feed, in particular to a yeast hydrolysate with high free nucleotide and a preparation method and application thereof. The yeast hydrolysate with high free nucleotide comprises protein, free nucleotide, amino acid and small peptide, wherein the content of the protein is 60-75% and the content of the free nucleotide is 35% or above based on the dry matter mass of the yeast hydrolysate, and the yeast hydrolysate is obtained through yeast milk enzymolysis, nucleotide acidification and nucleotide strengthening. The invention furtherprovides the preparation method of the yeast hydrolysate. The content of the free nucleotide in the prepared yeast hydrolysate is 35% or above, the preparation method is simple, and the obtained yeast hydrolysate is a natural product and is green and safe. The yeast hydrolysate with high free nucleotide can relieve the scarcity of a feeding nucleotide source, the breeding benefit is increased, and application prospects a