DNA bar code for identifying gerres lucidus and gerres filamentosus, identification method and applications
The invention provides a DNA bar code for identifying gerres lucidus and gerres filamentosus, an identification method and applications, and belongs to the technical field of molecular biology. The sequence of the DNA bar code of gerres lucidus is shown as SEQ ID No. 1; the sequence of the DNA bar c...
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Zusammenfassung: | The invention provides a DNA bar code for identifying gerres lucidus and gerres filamentosus, an identification method and applications, and belongs to the technical field of molecular biology. The sequence of the DNA bar code of gerres lucidus is shown as SEQ ID No. 1; the sequence of the DNA bar code of gerres filamentosus is shown as SEQ ID No. 2; and primers for amplifying the DNA bar code include 12S-FD and 12S-RD. The method includes the following steps: extracting the genome DNA of a to-be-identified sample; using the primers to amplify the to-be-identified DNA; performing comparison the sequence of an amplified product and the DNA bar code; and determining the species of the to-be-identified sample. Thus, stable results and strong repeatability can be achieved by utilizing the provided DNA bar code to identify the gerres lucidus and the gerres filamentosus.
本发明提供了一种鉴别边缘银鲈与曳丝银鲈的DNA条形码及其鉴别方法和应用,属于分子生物学技术领域,本发明所述边缘银鲈的DNA条形码序列如SEQ ID No.1所示;所述曳丝银鲈的DNA条形码序列如SEQ ID No.2所示。扩增所述的DNA条形码的引物,包括12S-FD和12S-RD;所述方法 |
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