Primer, probe and reagent kit for quick and quantitative detection by AR-V7 fluorescent qRT-PCR method

The invention discloses a primer and probe for detecting AR-V7 and ARE, and provides a reagent kit for detecting AR-V7 and AR based on the primer and probe. The reagent kit comprises an exosome extraction reagent, an exosome nucleic acid extraction reagent, an AR-V7 detection reagent by a qRT-PCR method. Samples for the reagent kit are broad and easy to obtain, the success rate for preparing exosomes is high, exosome nucleic acid is automatically extracted, the required operation time is short, and the operation errors are low. Besides, according to an one-step method qRT-PCR reagent providedby the invention, the operation flows are simplified, the experiment period is shortened, the efficiency is improved, and the sample contamination or detection fault risks in the middle process can also be reduced. 本发明公开了一种用于检测AR-V7及AR的引物及探针，并基于所述引物及探针提供了一种用于检测AR-V7及AR的试剂盒。该试剂盒包括外泌体抽提试剂、外泌体核酸抽提试剂和AR-V7的qRT-PCR法检测试剂。该试剂盒的样本来源广泛、获取容易，外泌体的制备成功率高，外泌体核酸采用自动化抽提，所需操作时间短，操作误差低。同时本发明提供的一步法qRT-PCR试剂，简化了操作流程，缩短了实验周期，提高了效率，也降低了中间过程样品