NOVEL PROTEIN PORES

The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like poreand a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications. 本发明涉及新颖蛋白孔及其在分析物检测和表征中的用途。本发明尤其涉及一种由CsgG样孔和经修饰的CsgF肽，或其同源物或突变体形成的分离的孔复合物，从而在纳米孔中并入附加通道收缩段或读取头。本发明还涉及一种跨膜孔复合物和用于产生所述孔复合物的方法，及其在分子感测和核酸测序应用中的用途。