Primer group for simultaneously detecting salmonella, pasteurella multocida, staphylococcus aureus and avibacterium paragallinarum and application

The invention provides a primer group for simultaneously detecting salmonella, pasteurella multocida, staphylococcus aureus and avibacterium paragallinarum, a kit and application, and relates to the technical field of multiplex PCR. The nucleotide sequences of the primer group disclosed by the invention are shown as SEQ ID NO: 1 to 8. The invention further provides the kit comprising the primer group and a detection method. Salmonella, pasteurella multocida, staphylococcus aureus and avibacterium paragallinarum can be specifically detected at the same time, and the specificity is good. The minimum detection amount of SE, PM and SA is 5*10ng/mu L. The minimum detection amount of APG is 5*10ng/mu L. The sensitivity is good. 本发明提供了一种同时检测沙门氏菌、多杀性巴氏杆菌、金黄色葡萄球菌和副鸡禽杆菌的引物组、试剂盒及应用，涉及多重PCR技术领域。本发明所述引物组的核苷酸序列如SEQ ID NO：1～8所示。本发明还提供了包括所述引物组的试剂盒以及检测方法，可特异性同时检测沙门氏菌、多杀性巴氏杆菌、金黄色葡萄球菌和副鸡禽杆菌，特异性良好，且SE、PM、SA最低检出量均为5×10ng/μL，APG最低检出量为5×10ng/μL，敏感性良好。