Recombinant vector, strain, and expression and purification method of Rhizoctonia solani effector protein

The invention discloses a recombinant vector, a strain, and an expression and purification method of a Rhizoctonia solani effector protein, and belongs to the biotechnical field. The recombinant vector is used for expressing the Rhizoctonia solani effector protein; the recombinant vector is obtained by inserting a coding gene of the Rhizoctonia solani effector protein into a position between EcoRIand XhoI restriction enzyme cutting site of a pET-32a vector; the amino acid sequence of the Rhizoctonia solani effector protein is represented by SEQ ID NO:1 in a sequence table; and the nucleotidesequence of the coding gene of the Rhizoctonia solani effector protein is represented by SEQ ID NO:2 in the sequence table. The strain with the recombinant vector is subjected to activation culture, isopropyl-beta-D-thiogalactoside is added to induce the expression of the target protein, the target protein is dissolved by using a protein dissolving buffer solution, and elution and dialysis treatments are carried out multip