Myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1, and application thereof

The invention discloses a myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 and an application thereof. The nucleotide sequence of the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 is as shown in SEQ ID NO. 1. According to the invention, a transc...

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Hauptverfasser: LI HAOHUA, LI SAINI, ZHU MUZI, CEN YOUFEI, YE WEI, LIU HONGXIN, ZHANG WEIMIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 and an application thereof. The nucleotide sequence of the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 is as shown in SEQ ID NO. 1. According to the invention, a transcriptome sequencing result is used to predict a gene sequence, a cDNA library is used as a template,and amplification is carried out to obtain the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1. The myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 can efficiently assist saccharomyces cerevisiae in resisting trichothecene toxins, and lay a molecular biological foundation for improving the trichothecene-resistant capacity of saccharomyces cerevisiae in the later period, improving the heterologous expression level of trichothecene toxins and obtaining novel trichothecene toxins. 本发明公开了一种露湿漆斑菌(Myrothecium roridum)A553抗单端孢霉烯自我保护基因mfs1及其应用。该基因的核苷酸序列如SEQ ID NO.1所示。本发明以转