Real-time fluorescent quantitative RT-PCR detection method for bovine norovirus

The invention relates to the technical field of molecular biology, in particular to a real-time fluorescent quantitative RT-PCR detection method for bovine norovirus (BNoV). According to the method, apair of specific primers is designed based on a targeted RdRp gene of the BNoV and a reaction system and reaction conditions of real-time fluorescent quantitative PCR are optimized, so that a fluorescent quantitative PCR method for detecting the BNoV is established. The method is good in linear relation and good in amplification efficiency; the sensitivity of the disclosed method is 1000 times ofthat of the conventional PCR method and is relatively high. The method only has a good amplification curve for BNoV, so that the specificity is high; and the difference in the groups and the difference between the groups are small and the high repeatability is realized. 本发明涉及分子生物学技术领域，具体涉及一种牛诺如病毒的实时荧光定量RT-PCR检测方法。该方法是基于BNoV的靶向RdRp基因设计一对特异性引物，并对实时荧光定量PCR的反应体系及反应条件进行优化，从而建立的检测BNoV的荧光定量PCR方法，该方法线性关系良好，扩增效率好；是常规PCR方法敏感性的1000倍