Method for separating and purifying N-(2)-L-alanyl-L-glutamine through nanofiltration membrane

The invention relates to separation and purification of N-(2)-L-alanyl-L-glutamine, in particular to a method for separating and purifying N-(2)-L-alanyl-L-glutamine through a nanofiltration membrane.A prepared N-(2)-L-alanyl-L-glutamine reaction solution is subjected to ceramic filtration enzyme removal and nanofiltration impurity removal and desalination in sequence to obtain a concentrated solution; the concentrated solution is evaporated into crystals, so that N-(2)-L-alanyl-L-glutamine with the purity of over 98% is obtained. According to the invention, the nanofiltration membrane is adopted to remove impurities and salt and the separation effect is improved, so that the N-(2)-L-alanyl-L-glutamine content in the concentrated solution obtained by separation is increased and the yieldis increased; the separation time is greatly shortened, the evaporation and concentration pressure is reduced, the energy consumption is reduced; the generated wastewater can also be processed to increase the yield of the N-(2