Method for detecting Erysipelothrix rhusiopathiae and primers and probe thereof

The invention belongs to the field of molecular biological detection and discloses primers and a probe for detecting Erysipelothrix rhusiopathiae. The sequences of the primers and the probe are as follows: F-primer: 5'-CCGAATTTGCTTACCGCTTGAAGGTTAAAGCAGGC-3', R-primer: 5'-GTCCAAATTGAAT...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN XUAN, LUO BAOZHENG, HUANG HAICHAO, ZHAO FUZHEN, LIAO XIUYUN, SHAO JIANHONG, SHA CAIHUA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the field of molecular biological detection and discloses primers and a probe for detecting Erysipelothrix rhusiopathiae. The sequences of the primers and the probe are as follows: F-primer: 5'-CCGAATTTGCTTACCGCTTGAAGGTTAAAGCAGGC-3', R-primer: 5'-GTCCAAATTGAATAGTTTTCTATATACATAAGATC-3'; Probe: 5'-AGGTCTAGCGCAGATCGAGGGTAAGTACAATAATCTCCAAAAGATAA-3'. The probe is labeled witha fluorophore, a quencher and THF; the fluorophore and the quencher are both labeled on a T base, and the spacing between the fluorophore and the quencher is 1-5nt; the THF is arranged between the fluorophore and the quencher; the distance from the THF to the 5' end of the probe is greater than or equal to 30 nt; and the distance from the THF to the 3' end of the probe is greater than or equal to15 nt. The invention also discloses a detection method of Erysipelothrix rhusiopathiae using the above primers and the probe. The detection method of the invention has strong specificity, high sensitivity and good stability, a