Protein extraction method and application

The invention provides a protein extraction method and an application, and particularly relates to a method for extracting protein from paraffin-embedded tissues and an application. According to the method, toxic xylene is replaced by a non-toxic TO transparent agent, so that the method is safe and environment-friendly; the protein is subjected to extraction treatment by using a high-temperature boiling method, so that low-abundance protein degradation and loss caused by tissue freeze-thaw cracking or ultrasonic crushing of a conventional operation is avoided; an antigen repairing step of an antigen repairing liquid adopting microwave heating is replaced by using metal bath heating; and by virtue of match of a nest screen cloth cell, the operation is simple and feasible, and the integrityof a tissue slice can be better guaranteed. The method not only improves the extraction amount of the total protein of the paraffin tissues, but also increases the detection sensitivity to the low-abundance protein; and the o