Recombinase polymerase amplification (RPA) primers and method used for detecting bacillus anthracis

The invention relates to the technical field of biology, and specifically discloses recombinase polymerase amplification (RPA) primers and a method used for detecting bacillus anthracis. According tothe RPA primers used for detecting the bacillus anthracis, 1 pair of RPA primers are designed and synthesized on basis of a conservative sequence of a bacillus anthracis BA5345 gene; and the RPA primers are shown as SEQ ID NO. 1-2. Subjected to further optimization on primer concentration, reaction temperature and reaction time, the bacillus anthracis can be specifically detected after addition of2.0 microliters of the upstream primer and the downstream primer, of which the concentration is 10 mmol/L, into 50 microliters of a RPA reaction system and subsequent constant-temperature reaction ina water bath pot at 39 DEG C for 30 minutes; and more RPA amplification products can be obtained. Thus, the RPA detection method developed by the invention has an sensitivity which is 100 times higher than sensitivities of con