Methods of inducing pluripotent adipose-derived stem cells

The invention discloses a method for inducing pluripotent adipose-derived stem cells. Ganoderma resinaceum element F is applied in the culture process. The method comprises the following steps: (1) carrying out isolated culture on rat ADSCs; and (2) osteogenesis induced differentiation: taking third-generation ADSCs cells with a good growth state, performing conventional digestion, terminating digestion, centrifuging and cell resuspending, inoculating the obtained cells into a six-pore plate with preset cover glass in a form of 100/pore, changing an experimental group into an osteogenesis induction culture medium 1 for osteogenesis induction culture for 1 day after the cells are completely attached to the walls, changing a liquid on the second day, changing into an osteogenesis induction culture medium 2, and continuing osteogenesis induction for 7 days. According to the invention, the ganoderma resinaceum element F is innovatively applied to inducing pluripotent adipose-derived stem cells to be rapidly and e