Preparation method and application of horse-derived anti-canine-parvovirus immune globulin

The invention provides a preparation method of horse-derived anti-canine-parvovirus immune globulin. The method comprises the following steps: firstly, preparing canine parvovirus immunogen; inoculating the canine parvovirus immunogen to a healthy horse; obtaining horse plasma and extracting immunoglobulin in the horse plasma; and finally, obtaining the horse-derived anti-canine-parvovirus immuneglobulin by means of purification and ultrafiltration and the like. The horse-derived anti-canine-parvovirus immune globulin prepared by the invention has a remarkable immune protection effect; a horse source instead of a homologous animal is adopted, so that the problem that the immunoglobulin extracted from the homologous animal can introduce exogenous viruses is solved; and meanwhile, the yieldof the immunoglobulin can be increased, and the purpose of large-scale production of the immunoglobulin preparations is achieved. 本发明提供了马源抗犬细小病毒免疫球蛋白的制备方法，该方法首先制备犬细小病毒免疫原，然后将其接种至健康马，收获马血浆，提取马血浆中的免疫球蛋白，最后通过纯化，超滤等手段获取马源抗犬细小病毒免疫