Detection kit for anti-cyclic citrullinated peptide antibody and preparation method of detection kit

The invention relates to a detection kit for determining the concentration of an anti-cyclic citrullinated peptide antibody and a preparation method of the detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a first buffer solution, sodium chloride, a first active agent, a first preservative and a first stabilizer; and the reagent R2 comprises a secondbuffer solution, sodium chloride, a second active agent, a second preservative, a second stabilizer and a latex microsphere coupled with CCP antigen-BSA. The latex microsphere of the kit of the present invention adopts the latex microsphere coupled with the CCP antigen-BSA, firstly, a CCP antigen is bound with BSA through a peptide bond, a CCP-BSA conjugate is formed, and the CCP-BSA conjugate iscoated on latex particles, so that a problem of low efficiency of direct coupling of the CCP antigen and the latex particles is solved. Secondly, abundant carboxyl groups are contained on the surfaceof a BSA molecule, so that