Protein and polypeptide protection agent and application thereof

The invention discloses a protein and polypeptide protection agent and application thereof, and aims at providing the target-protein (or polypeptide) protection agent during fermentation in the fieldsof genetic engineering and biopharmaceutics. The protein and polypeptide protection agent belongs to...

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Bibliographische Detailangaben
Hauptverfasser: GUO ZHIHUI, YUAN WANJUN, REN XIAOFEI, XU MINGBO, NIU GANG, QIN JINGCHAO, YUAN DELU, TAO ZHENMIN, LI YANG, CHEN JINGJING, SHU YANPING, LI ZHAOYING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a protein and polypeptide protection agent and application thereof, and aims at providing the target-protein (or polypeptide) protection agent during fermentation in the fieldsof genetic engineering and biopharmaceutics. The protein and polypeptide protection agent belongs to a polypeptide, and an amino acid sequence of the protection agent is (-Arg-Gly-)X and is abbreviated as (RG)X, wherein X=2, 3, 4, 5 or 6. The protein and polypeptide protection agent can be prepared by adopting a solid-phase synthesis method. The protein and polypeptide protection agent has effects of being capable of inhibiting hydrolysis of recombinant proteins or polypeptides which contain amino acid sequences of -Arg-Gly- or are easily hydrolyzed by proteases at Arg sites, promoting successful expression of target products, increasing the expression quantity of the target products, and reducing the content of related impurities. The protein and polypeptide protection agent can also reduce purification difficul