Sulfa-drug half-antigen, artificial antigen and application thereof in immunodetection

The invention discloses a sulfa-drug half-antigen, an artificial antigen and an application thereof in immunodetection and belongs to the field of immunodetection. A structure of the half-antigen hastwo aminobenzene sulfonamide structures, so that the prepared artificial antigen has a more outstanding spatial structure, the presenting of a common characteristic group aminobenzene sulfonamide of sulfa-drugs is better facilitated, and the immunogenicity of the antigen is improved; the artificial antigen is combined with an ELISA detection technology, and an IC50 value of sulfadimidine is 0.5 microgram per liter; and a sulfa-drug colloidal gold immunochromatography test paper strip prepared by using the artificial antigen can implement rapid and accurate detection. 本发明公开了一种磺胺类药物半抗原、人工抗原及其在免疫检测中的应用，属于免疫检测领域。半抗原结构中具有两个胺基苯磺酰胺的结构，使得制备而成的人工抗原的空间结构更突出，更有利于呈现磺胺类药物的共有特征基团胺基苯磺酰胺，提高了抗原的免疫原性，将人工抗原结合ELISA检测技术，磺胺二甲嘧啶的IC50值为0.5μg/L；使用本发明制备的磺胺类药物胶体金免疫层析试纸条，可以实行快速、准确的检测。